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The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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The native state of HuBChE is also stabilized by the kosmotropic sodium phosphate buffer; its flexibility is reduced and its resilience increased over a large temperature range as established by neutron scattering (44)
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Since we use relative peak positions and distance (inter-arm proximity) changes in our discussion here (and not absolute distances), variations of [gamma] from 1 do not affect the discussion or conclusions of this study
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