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. All spectra were corrected for background contribution by subtracting appropriate blanks containing only buffer and GdnHCl
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. Spectra were collected from 30°C to 98°C at intervals of 2°C with a spectral resolution of 4 cm^sup -1^ and 256 scans
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. Since ACYI can maintain its dimeric structure at a high GdnHCl concentration of 2 M (18), it seems that the dimerization domain was quite stable against denaturation
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. Similarly, the free energies of unfolding of C^sub H^2 antibody domain and pliage P22 coal proteins are, respectively, 3
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. This is the case if at least one major component of the surrounding solvent is chemically equivalent to the main repeating unit of the polymer making chain-solvent contacts preferable to chain-chain contacts (4,37)
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. Third, these increased fluctuations could certainly lead to the occasional close approach of distal amino acids
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. Structure of EI^sup SC^ by FTIR (steady-state measurements) In Fig
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. The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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. However, a direct correlation of the dynamical transition of fast, thermal fluctuations with the onset of enzymatic activity has been questioned (8)
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. 5), there is some correspondence between the measured and expected values of η^sub r,0^
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. Information on the dynamics of protein hydration may be obtained from ^sup 17^O magnetic relaxation dispersion measurements, which indicate that water molecules at protein surfaces are highly mobile (66,67)
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. Picosecond studies of the fluorescence probe molecule 8-anilino-l-naphthalenesulfonic acid
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