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Other big donors listed in the Carter Center's annual reports include the Sultanate of Oman and the sultan himself; the government of the United Arab Emirates; and a brother of Osama bin Laden, Bakr BinLadin, "for the Saudi BinLadin Group
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. The use and misuse of FTIR spectroscopy in the determination of protein structure
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. The first is a local length scale that spans seven-residue stretches
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. Direct test of the Gaussian-chain model for treating residual charge-charge interactions in the unfolded slate of proteins
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. The far-UV CD spectrum of EI^sup sc^ at neutral pH showed the features of an α-helical protein with minima at 208 and 222 nm (41,42) (Fig
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. With respect to all α-amylases investigated here, TAKA is the only protein which does not show a visible aggregation upon thermal unfolding (17)
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. LADH was purchased as crystalline suspension from Fluka Chemie GmbH (Buchs SG, Switzerland)
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. Independently from producing evidence for the step-resolved character of the process, intermediate and morphologically heterogeneous forms were trapped and characterized, which yields direct evidence for the multipathway character of the amyloidogenesis of insulin
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. 23 kJ K^sup -1^ mol^sup -1^
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. In contrast, the SL 4WJ shows a dramatic shift from very low E (0
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.0% glucosamine) of the mass of the lectin (16
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. Sharmistha Sinha* and Avadhesha Surolia*[dagger] * Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India; and [dagger] National Institute of Immunology, New Delhi, India Submitted July 3, 2006, and accepted for publication August 29, 2006
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. Denatured states figure prominently in a variety of studies on proteins especially as reference states for estimating protein stability (4,5,13-18)
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. Cavity statistics, P^sub oa^(r) folded ubiquitin are shown in Fig
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. Protein folding, stability, and solvation structure in osmolyte solutions
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. In EI^sup sc^, although its sequence homology and its structure are similar to those of other EI proteins, the calorimetric equilibrium unfolding, as shown by DSC, is different
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. The effect of protein concentration and heating rates It is well known that the kinetics and the extent of aggregation often depend not only on heating rates and temperatures but also on protein concentration (9,35)
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.5 T/m is required
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. Cross-linking of samples after incubation times >2 h showed a protein band that was unable to enter the gel, indicating formation of very large aggregates >1000 kD (data not shown)
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.Misfolding and aggregation kinetics monitored by ANS, Bis-ANS, DCVJ, and ThTTime course changes accompanying TTR misfolding and aggregation were recorded to elucidate preferential fluorescence signals of the probes depending on the conformational state of the protein
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. DCVJ responds to early species, i
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.Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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.01 kJ K^sup -1^ mol^sup -1^ (solid line in Fig
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