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. The conformational stability and structure of the EI from Streptomyces coelicolor, EI^sup SC^, were explored in the absence and in the presence of its effectors by using several biophysical probes (namely, fluorescence, far-ultraviolet circular dichroism, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry) and computational approaches
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. FoId-X was also used to evaluate the interaction energy in the dimer formed by both C-terminal domains by calculating the difference in stability energy between the dimer and the isolated domains
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. At the moment, however, we do not know whether these small changes in the structure are also responsible for the observed considerable specificity of the phosphotransfer reaction between EI and HPr among the different species and the differences in their cnthalpic variations in their binding reactions (45
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. Therefore, in this case, R-type flux having solder-flux interfacial tension 445 mN/mm (which is higher than the other two fluxes) contributes to the lower values of γ^sub LF^ - γ^sub SL^ and is attributed to the higher contact angles and negative wetting forces (Fig
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