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. That the intermediate is monomeric in nature is also evident from its fluorescence emission λ^sub max^, which is similar to its fully folded monomeric state, characterized earlier (23) (Fig
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. The PH domains of the enzymes are responsible for their different membrane binding characteristics; PLCδ^sub 1^ and its isolated PH domain binds specifically to membranes containing its substrate PI(4,5)P^sub 2^ through a series of specific hydrogen and ionic bonds (Ferguson et al
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. In that sense, the role of nonnative interactions in determining the type and rate of folding pathways is not a new idea (Zhou and Karplus, 1999)
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