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. Several functions for MG intermediates have been proposed, including binding to chaperones for correct folding (Bochkareva et al
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. If a fish living in a surface stream suffers a mutation harming its vision, it is more likely than its sighted fishmates to die of hunger or to be eaten by predators
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. The tetrameric enzyme has nine glycosylation sites per monomer, and carbohydrates account for ~25% of its dry weight
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. Moreover, residues with significant exchange broadening are found
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. The exchange broadening must then be due to conformational exchange between different conformations of bound CaM
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. Influence of the nature of the donor particle interaction with the receptor
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. When comparing the melting rates of the three polymers, the melting temperatures should be considered
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. In the diffusion spFRET measurements described in this article, by labeling the ends of the loop-bearing arms with donor and acceptor dyes, we use the FRET efficiency E as an indicator of the distance between arms A and B of the ribozyme during the docking process
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. The peak positions for the SL 2WJ and 3WJ constructs closely follow the trends observed for the undocked peak with the corresponding WT constructs, confirming that removal of the loop on arm A provides appropriate models for the undocked states of the WT ribozymes
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- Piper Professor of Biblical Theology at Princeton Theological Seminary
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. Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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