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. The crewmen, veterans of years of work on Lane's boats, eviscerate the fish and clean them with a hose before lowering them into the hold in canvas baskets
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. The reference solutions corresponding to N, A, and UA states were prepared with HCl into water as those pH values become equal to 5
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. Each of the subunits is glycosylated at the Asn-75 position (Fig
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. Denatured states figure prominently in a variety of studies on proteins especially as reference states for estimating protein stability (4,5,13-18)
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. The hydrodynamic radii (R^sub h^) for denatured proteins scale with chain length (N) as: R^sub h^ = 2
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. For comparison, the δ values calculated from native structures are also shown as open circles
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. Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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. Polymer coils with excluded volume in dilute solution: The invalidity of the model of impenetrable spheres and the influence of excluded volume on the rates of diffusion-controlled intermolecular reactions
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. Modeling unfolded proteins of peptides and proteins
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. 0) to find the optimum alignment (a 40% identity and a 52% similarity)
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. A dependence on the number of the glycosylation sites was found for only a small percentage of the exchangeable amide H-atoms (65), the exchange rate decreasing with increasing glycosylation
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. The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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. Three curing temperatures (150, 175, and 200°C) and three curing times (1, 2, and 3 minutes) were considered
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- These indicators, as well as other indicators such as write-offs attributable to denials, denials by payer, and bad debt, enable us to identify opportunities for improvement
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. The stability of the monomer of rSBA is comparable to many monomeric proteins of similar size
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. Being at the noncanonical interface, these glycans must be profoundly involved in holding the tetrameric structure rather than being involved in the folding of individual monomeric unit
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. Fluorescence spectroscopy provides a very attractive alternative for such studies, due to sensitivity, ease of use, and flexibility
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. However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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. Mean ([+ or -] SE), range and number ofobservations are shown in the table
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