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. We also determined the pressure range in which the proteins remain membrane bound: PLCβ binding was stable throughout the 1-2000 bars range, Gβγ binding was stable only at high membrane concentrations, whereas Gagr;^sub s^(GDP) dissociated from membranes above 1 kbar
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. fisheries can no longer meet demand; more than half our seafood is imported from all over the globe
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.Hydration of apomyoglobin in native, molten globule, and unfolded states by using microwave dielectric spectroscopyKamei, TakashiABSTRACT The high resolution dielectric spectra of semidilute solutions of apomyoglobin in native (N, pH = 5), acid-- induced molten globule (A, pH = 4), and unfolded (U^sub A^, pH = 3) states have been measured in the range from 0
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. Finally, the solution was dialyzed against TBS (Tris buffer (pH 7
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. EIN shows a reversible thermal two-state transition; whereas EIC has a broad-scan-rate, concentration-dependent thermal transition (2,12)
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. In contrast, RhoA induces the formation of stress fibers, focal adhesions, and cell spreading
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. This is in agreement with MD simulation data: Modifications in the dynamics of large protein complexes relative to their component subunit are dominated by low-frequency collective modes which take place on the 10-ps range upwards (2,49), and may be invisible in the experimental space-time window chosen
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. High pressure dissociated PLCβ-Gβγ with a ΔV = 34 ± 5 ml/mol
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. The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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. 1, A and B; curve 1, Fig
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., light scattering) or selectively detecting the amyloid fibrils (fluorescence of amyloids labeled with thioflavin T; e
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. It is generally accepted from thermodynamic and biochemical data that the β-sheet of gp120 that bridges the inner and outer domain of the gp120 core, the bridging sheet, is only fully formed and stabilized upon complexation with CD4 (Fig
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. T^sub g^ and initial modulus were determined by dynamic mechanical analysis (DMA, Seiko SII DMS 210)
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. For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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. Other projects supported by the group include psychiatrist Charles Grub's psilocybin research with terminal cancer patients at UCLA; Charleston, South Carolina, psychiatrist Michael Mitohefer's study of MDMA as a treatment for posttraumatic stress disorder; and University of Arizona at Tucson psychiatrist Francisco Moreno's study of psilocybin as a treatment for obsessive compulsive disorder
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