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Seung-Cheol Lee,* Jee-Hyun Cho,* Daniel Mietchen,*[dagger][double dagger] Young-Sook Kim,* Kwan Soo Hong,* Chulhyun Lee,* Dongmin Kang,§ Ki Deok Park,¶ Byong-Seok Choi,|| and Chaejoon Cheong* * Division of Proteome Research, Korea Basic Science Institute, Yusung-gu, Daejeon 305-333, Korea; [dagger] Fraunhofer Institute for Biomedical Engineering, Department of Magnetic Resonance, Sankt Ingbert, Germany; [double dagger] University of the Saarland, Faculty of Physics and Mechatronics, Saarbrücken, Germany; § Chuncheon Center, Korea Basic Science Institute, Chuncheon, Kangwon-do 200-701, Korea; ¶ Gwangju Center, Korea Basic Science Institute, Gwangju 500-757, Korea; and || Department of Chemistry and National Creative Research Initiative Center, Korea Advanced Institute of Science and Technology, Yusung-gu, Daejeon 305-701, Korea Submitted September 1, 2005, and accepted for publication November 16, 2005
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For all samples and all measured temperature points, we observed no Bragg peaks corresponding to water or salt crystalline structures
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Data analysisAll spectra were analyzed using SYBYL software (TRIPOS)
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Several amyloidogenic in vitro assays employ acidic pH for generation of amyloid fibrils; however, ThT shows very modest fluorescence at acidic pH when bound to protofibrillar amyloid of TTR (Fig
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Such quality defects are also associated with the development of fat agglomeration in the liquid mix after homogenization
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Each of these has its own way of visualizing how the collapse of a random coil to a native globule can ever be accomplished in observable time scales, a problem pointed out long ago (Levinthal, 1969)
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For example, a nonnative attraction beween the helices irrespective of their axial orientation would favor a two-dimensional search out of contact before a three-dimensional search in contact as the structure of the five-dimensional search space
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The percentages of positive stations were 5
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Consistent with this hypothesis, previous trFRET studies have shown that the 4WJ stabilizes the tertiary structure of the hairpin ribozyme by reducing the entropie penalty for docking as compared with ribozyme constructs containing either a 2WJ or 3WJ (Klostermeier and Millar, 2000)
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