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. Considering the commercial importance of aminoacylase, the results herein might facilitate the optimization of the conditions for the industrial use of ACYI
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. Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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. DISCUSSION Structure of EI^sup sc^ in the absence of effectors We carried out studies at different pH using several spectroscopic probes to test whether the structure of EIC^sup sc^ changed
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. t] x t + [[epsilon]
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