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Sea scallops are not
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A novel subset of amidohydrolases and insights into the enzyme mechanism
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The Flory random-coil model has been a topic of intense debate with cases being made for and against this mean-field model as an accurate descriptor of denatured states (132-117)
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Experiments between pH 4 and 5
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Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding
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Strambini, unpublished data) provides additional evidence that the creation of these low affinity ANS binding sites is owed to perturbations of the protein fold associated with the frozen medium
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The role of dynamics in enzyme activity
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Study of the dynamics of the complex can give insights into this novel type of CaM:target interaction
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Residues 14, 19, 51, 55, 76, 92, and 118 show a relatively strong decrease in exchange rate upon change in temperature (f = 0
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5 there is significant protein dimerization
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Aggregation of acid unfolded TTR (23°C, 14 µM monomer concentration) was induced by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM
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The context and consequences of ocular injuries from air guns
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Birds beware: several veterinary drugs may kill scavengersS
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For example, people who abuse steroids increase their risk for having heart attacks at a young age
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A possible reason may be that being in an enriched environment had a positive influence on whether the monkeys took drugs
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"This becomes clear at Willingboro when you consider how the large hoppers, or counting cells, are loaded with individual drugs
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The types of stressors section is comprised of five categories (frustrations, conflicts, pressures, changes, and self-imposed)
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EVEN AT HOMESome 2
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presidents, from Franklin Roosevelt to Dwight Eisenhower to Jimmy Carter to George H
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6M($25K- 40K/150K+)117The Entrepreneur's 283/0$71K-79K($45K) Source417Renaissance Executive 39/0($46
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. Moreover, the existence of MG folded states in the unfolding of GR was indicated by HPLC studies (Fig
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. Further, a statistical analysis of reported eukaryotic glycoproteins in the literature shows that the exlent of N-glycosylation easily outnumbers O-glycosylation
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. We observe that the unfolding of gSBA is a two-state process
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. (11) used small-angle x-ray scattering (SAXS) to measure R^sub g^ as a function of N for 28 different chemically denatured proteins, with chain lengths varying from 8 to 549 residues
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. Thermal denaturation experiments were also carried out by following the emission fluorescence of ANS either at 480 or 520 nm, after excitation at 360, 380, or 390 nm, at three different pH, 2
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. Seung-Cheol Lee, Jee-Hyun Cho, and Daniel Mietchen contributed equally to this work
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. These substrates were then coated by flux and used for testing
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. Despite these features the overall interaction is highly specific with nanomolar binding strength (16)
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. 3 shows the theoretical RT^sup n^^sub 1^ curves for a molecule with a correlation time, τ^sup o^^sub m^, of 10 ns with one internal motion, τ^sub if^, ranging between 20 ps and 6 ns, and squared order parameter, S^sup 2^^sub f^, ranging between 0
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. Theoretically the occurrence of particle gel formation is linked with percolation although this is a necessary rather than a sufficient condition for gelation to occur (21)
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. Additional dehydration of monocations can occur inside DNA grooves as a result of deformation of DNA secondary structure
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. Insulin fibrils feature characteristics common to all amyloids: x-ray diffraction patterns; affinity to Congo Red and Thioflavin T; birefringence; biological dysfunction and high stability against temperature; pressure; low pH; and proteases
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. Since the diffusion is always maintained in some low-dimensional subspace of the full folding space, τ ~ R^sup 2^/D for each subspace, so that τ^sub fold^ ≈ d^sup 2^R^sup 2^/D rather than the exponentially larger (R^sup 2^/R^sup 2^^sub N^)^sup d^
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. The intermolecular hydrogen bond network and the insertion of CD4-F43 into its receptive cavity seem to predominantly determine the large entropy loss upon complexation
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