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. The width of the olfactory pit in blind fish increases on average by 13 percent, thus enlarging the surface of the olfactory epithelium, where the chemical receptors are lodged [see illustration on opposite page]
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. However, considerable structural changes were observed for ACYI when heated to 60°C (26)
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. This solution was then dialyzed against 1
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. Although the three-dimensional structure of these α-amylases show up rather similar, the corresponding stabilities differ significantly (16,17)
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. 586 µm for Sn-Pb and 0
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. The exchange data for all residues are summarized in Fig
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. The system was equipped with a TBX-04D picosecond photon detection module with IBH laser diodes operating at 403 and 443 nm giving 150 ps pulses, as well as an IBH NanoLED operating at 373 nm giving 1
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. Some residues show positive intraresidue entropy change upon complexation
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. , Microporous Membrane Formation via Thermally-induced Phase Separation, III: Effect of Thermodynamic Interactions on the Structure of Isotactic Polypropylene Membranes, J
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.To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig
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. The results are shown in Fig
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. For instance, we have shown that the thennodynamic folding parameters (ΔC^sub p^ and m-values) of the HPr of S
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. However, we cannot rule out that an alternative explanation to the titration curve is that the deprotonation of a histidine residue can be felt by a residue(s) far enough in the three-dimensional structure (see Fersht (45) and references therein) affecting its (their) environmcnt(s)
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. Furthermore, the shape of the CD spectrum was altered between pH 3
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. The highly purified HuBChE was stored at 4°C after lyophilization from 10 mM sodium phosphate, pH 8
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.Of course, it is not impossible to achieve the dimensional reduction we have outlined by using fully native, rather than nonnative interactions
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