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. Lately, a study by Trp phosphorescence did provide direct evidence that the solidification of water causes important alterations of the native globular fold in all protein examined (3)
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. Non-denaturing gel electrophoresis of lipid-free and DMPC-associated proteins was carried out by using 8-25% polyacrylamide gradient gel (PHAST system, Pharmacia, Peapack, NJ) with Coomassie staining
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