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Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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When compared with the EIC^sup tt^
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However, there are examples of homologous proteins that unfold following different patterns and, in addition, have different thermodynamical parameters (60,61)
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The transient nature of oligomeric intermediates of misfolded TTR that later mature into fibrillar aggregates makes them hard to study, and methods to study these species are sparse
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Similarly, repetitive T-jumps with DMPC disks containing apoA-1 and apoC-1 show faster unfolding in the second and consecutive jumps (data not shown)
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Interestingly, the activation energy E^sub a^ = 60 ± 8 kcal/mol of the slow heat-unfolding phase in apoA-2:DMPC disks (Fig
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This is usually interpreted to mean that the residue in question enjoys the majority of its native contacts at the transition state (Fersht, 2000)
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Samples were fixed in a solution of 5% formalin to seawater
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Reactivity (%)EggSpecies (positive egg no
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