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. Finally, proteins-which represent one-third of the oocyte's dry mass, far exceeding the contribution of ribonucleic acids (41)-have been shown to bind large quantities of diminazene in other systems (see Mamman et al
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. Since soman inhibition is known to stabilize the folded state, this implies that the soman-inhibited unfolded state
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. Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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. The loss of these specific tertiary docking interactions makes these SL constructs mimics for the undocked state of the corresponding WT ribozymes
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. Since spectral shift measurements showed a sharp transition between 1 and 3 M GdnHCl (Fig
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. At 3 M GdnHCl, however, a significant drop in ellipticity was observed
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. Finally, we studied the conformational and stability properties of EI^sup sc^ in the presence of the effectors (i
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. (32) The first pathway includes UNC-73, MIG-2, ced-12, ced-5, and ced-2 (representing the mammalian homologues TRIO, RhoG, ELMO, Dock 180, and CrkII, respectively)
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.Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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