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. Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins
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. The appearance of the unusual exothermic peak and the lack of endothermic peak suggested that the unfolding of ACYI was accompanied by serious aggregation, which might be a nucleation-dependent process (36)
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. 5 B clearly indicates that the change of the band at 1628 cm^sup -1^ was the dominant event at temperatures below 66°C, whereas the asynchronous plot was dominated by noise and no crosspeaks could be identified at a similar position in the synchronous plot
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. Attributes of Glycosylation in the Establishment of the Unfolding Pathway of Soybean AgglutininSinha, SharmisthaABSTRACT Soybean agglutinin (gSBA) is a tetrameric legume lectin, each of whose subunits are glycosylated
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. For example, scaling of chain size with chain length can provide a direct probe of the nature of chain-solvent interactions (37,38,42,44,45)
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. In Protein Folding Handbook, Vol
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. This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig
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. The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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. Our results also highlight the ability of the diffusion spFRET method to resolve conformational subpopulations important during the course of RNA folding
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