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. Unfolding of GR by GdnHCl was further characterized by intrinsic fluorescence anisotropy measurements (Fig
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. Details with regard to the intrinsic fluorescence experiments were the same as those described before (30)
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. For example, the calculated ratio of k^sub cat^ (DmAChE/HuBChE) for hydrolysis of various substrates at 25°C is 1
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. 2 shows the unheated insulin sample, which at room temperature (20°C) has little tendency to aggregate
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. Hairpin ribozyme activity assayThe substrate strands S2WJ, S3WJ, and S4WJ were labeled radioactively with ^sup 32^P at the 5' terminii
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