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Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: a fluorescence spectroscopic analysis
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A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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Wiley-VCH Verlag, Weinheim, Germany
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We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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The S^sup 2^^sub s^ was either kept at a uniform constant value (M2I) or optimized together with τ^sub if^ and S^sup 2^^sub f^ (M2II)
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Nevertheless, the established fast exchange between bound and free CaM does not exclude the possibility of slow exchange between one (or more) highly populated bound state(s) of CaM and a lowly populated bound state
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To further demonstrate the use of fluorescence anisotropy decay, we probed TTR aggregation kinetics using time-resolved anisotropy
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Structural mapping of an aggregation nucleation site in a molten globule intermediate
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This is not yet reachable for proteins with the current computation timescale of nanoseconds
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, Studies on Toner Properties and Fabric Performance Properties for Xerographic Textile Printing, Masters thesis, Georgia Institute of Technology, Atlanta, GA, 1996
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Response variables = AF and RF,treatment factors = year, and covariate = age
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