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. Interestingly, similar results are obtained for the protein modeled as a fully extended conformation (Fig
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. Persistence of native-like topology in a denatured protein in 8M urea
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. Experiments were carried out at pH 2
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. It is however notable that the ThT molecule also responds to early oligomers (Fig
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. Similarly, repetitive T-jumps with DMPC disks containing apoA-1 and apoC-1 show faster unfolding in the second and consecutive jumps (data not shown)
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. These data were well approximated by mono-exponential functions, and the Arrhenius analysis yielded the activation energy E^sub a^ = 50 ± 5 kcal/mol, consistent with our estimate based on the scan rate effects on the melting data (Fang et al
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. For the WT ribozymes, docked fractions (F) were calculated as a ratio of docked/undocked areas (A) as, F = A^sup docked^/(A^sup docked^ + A^sup undocked^)(Deniz et al
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