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. He also served as a financial analyst at both Ohio Savings Bank and National City Corporation both located in Cleveland, Ohio
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. However, the order of events from Fig
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. Thus the β-sheet on the surface of the catalytic domain, Which is exposed to water in native ACYI, might unfold first
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. All α-amylase structures bear multiple tryptophan residues more or less equally distributed over the whole structure (see Table 1 and Fig
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. Thus, removing an interaction is indeed expected to have only a small effect on the affinity
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. The preparation contained approximately equal amounts of BLG A (mass 18365) and BLG B (mass 18278)
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. 9 A it is clear that it is more rapidly tumbling than the native form as indicated by the initial slope near t = 0
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. Additionally, the scan-angle was changed in cases of repeated scans
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. This algebraically much larger design-space for the search pathway corresponds to the more numerous set of planes that carry stabilizing interactions in a high-dimensional space than the set of coordinate planes alone (these, we recall, correspond to the use of native interactions alone)
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. The sections were subsequently incubated with the selected primary antibody for 30 minutes at room temperature
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. The cells cultured in DMEM/ 10%FCS were associated with reduced extracellular matrix, as indicated by toluidine blue staining, when compared with pellets maintained in DMEM/ITS+/TGF-[beta]1, which stained metachromatically (Fig
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. These folding intermediates exhibited flexible tertiary structures and nativelike secondary structures
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. In this research, the sequential events in ACYI thermal unfolding were investigated by a combination of spectroscopic methods and the related resolution-enhancing techniques
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. The two positive peaks located at 1618 and 1682 cm^sup -1^ confirmed the appearance of the aggregates observed in Fig
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. This scaling ensures that claims of persistent, long-range contacts are not predicted by theories for polymers in the EV limit
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. This inherent weakness of the Flory mean-field theory also demands extreme caution when extrapolating simulation or experimental results from peptides (10,12,43,58,101,102,129,138) to draw conclusions about denatured proteins
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. A similar behavior, which is typical for most proteins (20), is also observed for TAKA, BSUA, and BAA (see Table 2)
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.Longitudinal (R^sub 1^) and transverse (R^sub 2^) ^sup 15^N-spin relaxation rates were measured at 400 and 600 MHz ^sup 1^H frequency (40
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. Using polyethylene glycol solutions as a model of a crowded cell environment, we studied DNA compaction by alkali metal salts LiCl, NaCl, KCl, RbCl, and CsCl, and found that all of these monocations promote DNA compaction
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. These data were fitted with a cooperative binding model, and the resulting fits are shown as solid lines in Fig
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