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. thanks the Council of Scientific and Industrial Research, India, for the award of Senior Research Fellowship
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. It is obvious that this also can have different effects on the microenvironment of tryptophan residues
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. On freezing of the solution, however, the protein sharply enhances the probe fluorescence and shifts its spectrum to the blue
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. 02ns, S^sup 2^^sub f^ = 0
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. Thus, the dynamic features presented here support the conclusion that the conformationally heterogeneous SEF2-1mp, trapped inside the CaM dimer, constantly exchanges between different binding sites
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. To our knowledge, this study represents the first application of pressure to membrane-bound proteins under conditions where the proteins are bound to the membrane surface
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. Circular dichroism (CD) and light-scattering melting curves show hysteresis and scan rate dependence, indicating thermodynamically irreversible transition with high activation energy E^sub a^
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. The main TIPS parameters are polymer and solvent types, polymer mass fraction, polymer molecular weight, and solvent molecular weight
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. and 1 1 did not yield a sigmoidal behavior (data not shown)
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. Rho GTPases are molecular switches, cycling between inactive (guanosine diphosphate-bound) and active (guanosine triphosphate-bound) configurations
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. The R^sub 1^, R^sub 2^ and ^sup 15^N-{^sup 1^H} NOE data are presented in Fig
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. Membrane binding of phospholipases C-β^sub 1^ and C-β^sub 2^ is independent of phosphatidylinositol 4
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