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. 5 B clearly indicates that the change of the band at 1628 cm^sup -1^ was the dominant event at temperatures below 66°C, whereas the asynchronous plot was dominated by noise and no crosspeaks could be identified at a similar position in the synchronous plot
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. This erroneous model is of interest only because it has been used previously for denatured proteins in studies aimed at correlating m values and ΔC^sub P^ to changes in solventaccessible surface area (90)
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. Fluorescence measurements Fluorescence spectra were collected in a Gary Eclipse spectrolluorometer (Varian, San Carlos, CA) interfaced with a Pettier cell
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. This agrees with the DSC data from an identical sample indicating that disk reconstitution after heat denaturation takes more than 48 h to complete at 24°C (Fig
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. The advantage of the hypergutter approach, however, is that it identifies diffusive subspaces in cases where the standard diffusion-collision model does not
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. ProcedurePermission from the human subjects review board was granted to conduct the current study
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. At this pH the protein has a net negative charge, the dimer carrying a charge of -17e (36), where e is the protonic charge; and so the apparent specific volume would be expected to have a contribution from net repulsive electric double layer interactions in addition to the excluded volume of the hydrated protein itself
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.Thus, the effective size of the monocation in compact DNA is expected to be closer to the size of free ion without hydration shell, where the sequence of the monocation radius is Li+ CONCLUSIONOur investigation highlights the inverse relation between DNA compaction potentials of monovalent cations in a crowded environment and the previously reported DNA binding properties of different alkali ions
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