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In addition, size-exclusion chromatography analysis indicated that the intermediates populated at 2
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4 shows quenching of GR fluorescence by iodide
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An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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There are significant differences in the thermal stabilities of the two forms
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1 C), with a maximum shifted to lower pH (at pH 6) than that found in fluorescent experiments (Fig
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It can therefore be concluded that free and peptide-bound CaM are in fast exchange
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However, the sliding mechanism is usually a nonspecific target interaction, whereas in CaM-SEF2-lmp it is specific
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For proteins containing N residues, there are of order N^sup 2^ nonnative interactions that may be encountered during a diffusive search, but only of order N interactions that define the native state
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. Dielectric spectroscopy provides direct information of the dynamic behavior of molecular dipole orientation in solution
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. Equation 2 implies that when contact angle θ^sub c^ increases, the surface tension force moves toward negatives due to the cosine value of θ^sub c^, resulting in the negative wetting force
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. During interphase, DNA exists in a rather compact state (compared to the elongated state in aqueous solution) and extra compaction is required during mitosis to realize cell division
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. We have found that the emission intensity of labeled coumarin-PLCβ and coumarin-Gβγ showed a substantial and reproducible increase upon the addition of its unlabeled partner, which gives apparent K^sub d^ values similar to those obtained using FRET (Guo et al
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. In contrast, heating to 100°C, i
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.5 kJ K^sup -1^ mol^sup -1^ per atom, although gp120 shows an unexpected increase of 0
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. Standard dry and wet crockfastness tests were conducted (AATCC Test Method 8-1988) with ratings of 1 = entirely unacceptable, 2 = unacceptable, 3 = borderline acceptable, 4 = good, 5 = excellent (no color transfer)
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