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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate colorado home loan .
2 K; 4 µM, T^sub m^ = 329 colorado home loan .
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In principle, the protein-protein interface would be the same whether the protein is freely diffusing in solution or confined on a membrane surface, and thus we expect that for a specific association the volume change would be similar if the proteins were freely diffusing or confined to a membrane surface colorado home loan .
The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG* colorado home loan .
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The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements colorado home loan .
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