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Deterministic pressure dissociation and unfolding of triose phosphate isomerase: persistent heterogeneity of a protein dimer
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Consistent with previous observations (26), the intensity of the intrinsic Trp fluorescence decreased with the increase of temperature, and meanwhile, the emission maximum wavelength red shifted from ~333 nm to 340 nm (Fig
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6), although isothermal melt done with rSBA at this protein concentration still shows a three-state unfolding profile
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This trend is consistent with a pronounced binding capacity of oligomers whose subunits tend to dissociate in frozen aqueous media thereby making accessible large hydrophobic patches of the subunit interface
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Theory and range validation
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5 there is significant protein dimerization
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55% of the stations in densities that varied between two and 74 larvae/10 [m
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Mean ([+ or -] SE), range and number ofobservations are shown in the table
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This means that the affinity of gp120 for CD4 and CD4-induced monoclonal antibodies (CD4MAb) is relatively small, and can be fine-tuned by small variations in those two rather large contributions
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[12] prepared polypropylene particles by means of TIPS
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Samples for transmission electron microscopy were fixed in 0
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