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. In addition, the peak corresponding to tryptophan emission displayed a significant red-shift to [lambda]^sub max^ ~ 334-339 nm
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. The question we ask is, what is the probability that a sphere of radius a placed at random with respect to the center of mass of the chain will be empty? For each conformation in the EV-limit ensemble, we place a probe sphere of radius a at several random locations with respect to the center of mass and quantity the number of times a chain atom crosses the probe sphere
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. A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. Phase transition behavior of the isolated polymer chain
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. A similar behavior, which is typical for most proteins (20), is also observed for TAKA, BSUA, and BAA (see Table 2)
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