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Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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Nevertheless, Darwin was stumped over just how the same kind of mechanism could work for regressive evolution
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Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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These correlations lead to scale invariance in a variety of chain properties and direct evidence for correlations can be obtained by quantifying the scaling of internal distances
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42 kcal mol^sup -1^) and hydrophobic contacts (ΔΔG = + 4
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Other diseases are caused by a gain-of-toxic-function mechanism often including aggregation of misfolded proteins
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As an example of the type of fine-structure predicted, the model contains a natural explanation of the curvature seen in plots of the denaturant dependence of folding rates
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Since mass customization is extremely important in today's competitive world, it is commonly believed that digital printing will eventually replace the current methods of textile printing
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After the reproductive season, the ovaries of immature black rockfish once again became pale pink in color
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Regardless of the mechanism there should have been signs of greater atresia in the ovaries of young fish, which we did not observe in our histological preparations
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He and his coauthors caution that the method has a long way to go before it can be used in people
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He has since focused most of his energy in the gaming industry, although he still has business in real estate, and even has a company in Namibia, Africa, that converts General Motors cars from left-hand drive to right
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But the idea of mathematical probability theory did not take shape until the 1600s, when Blaise Pascal tried to help a friend who had asked for help toward beating the casinos
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One Blackjack MV-22B is, at this writing, in Canada for six months of icing tests
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This block effect was significant, F(7, 49) = 2
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The amount bet, the outcome, the amount won on that hand, and total winnings were displayed at the very bottom of the screen
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- All good cash management begins with a thorough assessment of your business's current cash position and the development of a forecast based on that, says Jonathan Gassman, a partner with accounting firm Gassman & Golodny LLP in New York City
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. There is also anecdotal evidence that sighted people have keener nonvisual senses when they cannot see--remember how the nonvisual senses seem to come alive in a cave when the lights go out? Would blind people reorganize their neural networks better than eyed people, if both groups began living in the dark or inside a cave? That is a question our students, and readers, will have to ponder
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. To verity the results obtained in Fig
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. Decomposition of protein tryptophan fluorescence spectra into log-normal components
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. We hypothesize that this compensatory effect could be due to a change in the coordination sphere of the Mg^sup 2+^ by directly involving the phosphoryl group of the PEP moiety, as it occurs in a member of the pyruvate dikinase family (57)
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. Structure and Mechanism in Protein Science
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. By assuming equilibrium conditions and an ANS activity in ice equal to the concentration in solution multiplied by a freeze concentration factor of 120 (12), one derives ANS dissociation constants in the range of 6-8 mM for WT, 2-4 mM for C3A/C26A, 2
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. Indeed, several lines of evidence indicate that both pressure-induced and thermal denaturation of HuBChE are multistep processes that are initiated by penetration of water into the active-site gorge, leading to protein swelling and, eventually, unfolding (18,53,54)
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. In solution, this helix is disrupted in the middle (2), which allows N- and C-terminal domains of both apo-CaM and Ca^sup 2+^/CaM to tumble almost independently of each other (3-5)
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. The exchange data for all residues are summarized in Fig
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.MATERIALS AND METHODSMaterialsBLG was obtained from Sigma-Aldrich (Poole, UK) (L0130) and is a mixture of genetic variants A and B
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. What is more unusual is the salting-in behavior which affects both the solid-liquid and the liquid-liquid coexistence lines
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. Thus, incubation of intact disks at 75°C for 30 min leads to disk fusion into enlarged particles that form stacks similar to disks but have larger diameters (d ≥ 25 nm) and occasionally form U-shaped bilayer structures typical of collapsed protein-containing vesicles (Fig
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. 7 B) and the melting data recorded by CD, light scattering, and DSC (Fig
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. Functional and immunological characterization of human-immunodeficiency-virus type-1 envelope glycoproteins containing deletions of the major variable regions
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