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. Contact order, transition slate placement, and the refolding rates of single domain proteins
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. Two invertebrate acetylcholinesterases show activation followed by inhibition with substrate concentration
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. Samples containing 4 x 10^sup 5^ cells were centrifuged at 500 g in 15 ml polypropylene conical tubes
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. The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein
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. An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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. The spectra were collected at a scan speed of 50 nm min^sup -1^
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. Consequently, in the EV limit, a range of chain properties show so-called scale invariance
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. Recently, Zhou (114) computed the functional form of x^sup 2^P(x) from EV simulations of proteins studied by Wilkins et al
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.1% of the residues in the most favorable regions and 10
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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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. Aggregation and denaturation of apomyoglobin in aqueous urea solutions
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. Protein denaturation during freezing and thawing in phosphate buffer systems: monomeric and tetrameric β-galactosidase
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.MSDs above 61°CThe finding that MSDs of native HuBChE tend to be larger than those of soman-inhibited HuBChE starting ~3° below the denaturation temperature of native HuBChE (~64°C) is in line with results from several techniques indicating that denaturation increases atomic flexibility of proteins (17,51,52)
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. Aggregation of acid unfolded TTR (23°C, 14 µM monomer concentration) was induced by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM
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. We find that pressure allows for determination of volume changes which give novel information about the interactions between proteins in the G-protein-PLC signaling system
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. It is fascinating to think that nature custom-made proteins so that, when digested by gut enzymes, useful bioactive peptides result
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., Liquid-liquid Phase Separation and Crystallization in Binary Polymer Systems, Polymer 28 (2), 193-200 (1987)
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. and plotted in the form of a histogram
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.Influence of helical junction geometry on docking efficiencyWe obtained FRET efficiency histograms for three ribozyme constructs with different helical junction geometries (natural 4WJ as well as corresponding 2WJ and 3WJ constructs; Fig
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