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. It is generally assumed that two distinct enzymes are involved in the intracellular degradation of N-acetylated proteins: the N-acetylated peptide hydrolase catalyzes the hydrolysis of the N-terminal amino acid residues from the N-acetylated proteins causing the release of acetylamino acids, which are finally hydrolyzed by ACYI (10,11)
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. Thus IR experiments were performed to further investigate the sequential events during the thermal denaturation of ACYI
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. 5 mM of PEP and 5 mM of Mg^sup 2+^
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. TTR is also associated with the sporadic disease senile systemic amyloidosis (Westermark et al
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. An estimation of cell number and viability was performed
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