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This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein chip free gambling .
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Several are located in the putative trimerization interface, S243, C378, and 1439, and two of them, V255 and L260, are located in the β-turn that connects the inner and outer domains of gp120 chip free gambling .
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