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. It has been studied in many aspects by using various methods
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. Accurate models for denatured states also impact a range of areas, including quantitative studies of in vitro folding pathways (10,19-24), protein design (25,26), studies of protein aggregation (27-29), and understanding preferential interactions in cosolute mixtures (1,3,30-36)
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. ) the ensemble of unfolded states is characterized by diverse structural and conformational properties
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. The fluorescence emission, collected at 90° from the excitation, is dispersed by a 0
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. It appears therefore that even for a homogenous insulin sample undergoing uniform temperature treatment, there is still more than one mode of superhelical assembly of protofilaments
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. Bonvin** Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands; and [dagger] Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, Zurich, SwitzerlandSubmitted April 23, 2004, and accepted for publication September 20, 2004
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. Since the folding process is complex in multidomain proteins, where each domain may be able to refold independently and interdomain interactions may affect the overall folding process, EI can be used, in addition, as a model protein to improve our knowledge of protein folding and assembly of multidomain-oligomeric proteins
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