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Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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The recomhinant form also shows emission maxima at 327 nm for the native stale and 357 nm in the completely denatured state (Fig
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Estefanía Hurtado-Gómez,* Gregorio Fernández-Ballester,* Harald Nothaft,[dagger] Javier Gómez,* Fritz Titgemeyer,[dagger] and José Luis Neira*[double dagger] * Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Alicante, Spain; [dagger] Lehrstuhl für Mikrobiologies, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany; and [double dagger] Biocomputation and Complex Systems Physics Institute, Zaragoza, Spain Submitted October 31, 2005, and accepted for publication March 7, 2006
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While efferocytosis was originally thought to be limited to "professional" phagocytes, such as macrophages and dendritic cells, we now know that most cell types in the body are capable of ingesting apoptotic cells, including epithelial cells and fibroblasts (Fig 1, bottom, B)
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Further and more detailed insights in the mechanisms of how aggregation affects properties of the unfolded state can be gained from studies where aggregation, in particular of thermal unfolded states, is suppressed
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