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. MethodsSamples for fluorescent microscopy were illuminated with 365 nm ultraviolet light, and fluorescence images of DNA molecules were observed using a Zeiss Axiovert 135 TV microscope equipped with a 100× oil-immersed lens and recorded on S-VHS videotape through a Hamamatsu SIT TV camera
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. S rangeYearIchthyoplankton surveys1978-79 42[degrees]27'-45[degrees]30'198235[degrees]55'-40[degrees]53'198338[degrees]30'-44[degrees]32'198544[degrees]41'-46[degrees]52'198641[degrees]34'-44[degrees]36'43[degrees]01'-46[degrees]50'199135[degrees]49'-36[degrees]51'199541[degrees]16'-45[degrees]22'199642[degrees]29'-44[degrees]01'199842[degrees]21'-45[degrees]36'199943[degrees]21'-44[degrees]01'200043[degrees]19'-46[degrees]24'200143[degrees]19'-46[degrees]54'42[degrees]54'-45[degrees]25'42[degrees]21'-43[degrees]42'Posttransition juvenile trawls199242[degrees]04'-45[degrees]43'199843[degrees]18'-47[degrees]02'199943[degrees]10'-47[degrees]01'200043[degrees]45'-47[degrees]02'200142[degrees]54'-45[degrees]25'Long
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. Three days after the final immunization, the spleens of the mice were removed and passed through a mesh (mesh size: 100 [micro]m)
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