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. We observe that the unfolding of gSBA is a two-state process
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. Furthermore, the spectroscopically investigated volume is to be taken into account-it is just (200 µm)^sup 3^ or 8 nl
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. The CaM binding site coincides with the basic DNA-binding sequence of the bHLH dimers (11,15,16,17)
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. Indeed, compaction potential appears to be inversely related to the binding activity of monocations
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. For typical membrane concentrations used in these studies, the apparent K(d) should be ~200-fold stronger than the true K(d) (see Runnels and Scarlata, 1999)
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. Other chemicals of suprapur or ultrapure grade were obtained from VWR (Merck GmbH, Darmstadt, Germany)
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. The excitation wavelength was 285 nm and emission was measured at 315 nm
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. In the common wrap-around CaM:target binding the hydrophobic patches of CaM and target fit well, resulting in an induced-fit binding where only one binding alternative is needed
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