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.5 kbar (data not shown), a pressure at which a large number of protein dimers and higher oligomers have been found to undergo subunit dissociation (Gross and Jaenicke, 1994; Heremans and Smeller, 1998)
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. Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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. This helix-loop-helix motif folded against the ban-el, covering a hydrophobic patch (which includes Tip^sup 461^): the motif was linked to the barrel by two salt bridges, namely, Arg^sup 537^-Asp^sup 467^ and Arg^sup 546^-Asp^sup 459^ (Fig
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. it is tempting to suggest that the sole peak observed in the thermal scans of EI^sup sc^ was due to the EIN^sup sc^
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. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2
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.The backbone structures of the CaM N- and C-terminal domains within the CaM:SEF2-1mp complex are expected to be very similar to those in other CaM structures
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. As shown in Fig
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. Anne Holbrook is the recipient of a CIHR Career Investigator Award; Adrian Levy is supported by a Scholar award from the BC Michael Smith Foundation for Health Research
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.ProcedurePermission from the human subjects review board was granted to conduct the current study
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