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For all its up-tempo numbers, the concert is shot through with deep melancholy that seems to have clicked with a literally captive audience
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. Theoretical studies of protein folding
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. INTRODUCTIONSignal transduction through heterotrimeric G-proteins is initiated by the binding of an extracellular ligand to its corresponding G-protein coupled receptor (for background, see Alberts et al
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. These enzymes deplete the polar HDL surface of the proteins or phospholipids and/or increase the apolar lipid core, thereby creating a mismatch between the particle surface and its core (clay et al
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. (16) This exposure of PS by apoptotic cells is of particular interest, because it induces membrane ruffling and efferocytosis, and it has been implicated in many of the immunomodulatory effects associated with efferocytosis (more below)
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. ANS binding experiments, extended to a set of proteins including serum albumin, α-amylase, β-galactosidase, alcohol dehydrogenase from horse liver, alcohol dehydrogenase from yeast, lactic dehydrogenase, and aldolase, confirmed that a stressed condition of the native fold in the frozen state appears to be general to most proteins and pointed out that oligomers tend to be more labile than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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. 3, A and B, are characteristic of the latter protein class
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.The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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. For prolate ellipsoids of this aspect ratio, the maximum packing fraction is ~0
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. The binding affinity was compared between the different probes defining an intensity 50 value, I^sub 50^, being the half height of the maximum emission intensity, corresponding to an apparent dissociation constant
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. Kluwer Academic/Plenum Publishers, New York
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. We also determined the pressure range in which the proteins remain membrane bound: PLCβ binding was stable throughout the 1-2000 bars range, Gβγ binding was stable only at high membrane concentrations, whereas Gagr;^sub s^(GDP) dissociated from membranes above 1 kbar
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. PLCβ contains the N-terminal PH domain of PLCβ^sub 2^ and the catalytic domain of PLCδ^sub 1^ (Wang et al
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. High-resolution nuclear magnetic resonance studies of proteins
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.ApoA-1, apoA-2, and other exchangeable apolipoproteins have evolved from a common ancestor that is related to the smallest human apolipoprotein, apoC-1 (57 aa) (Luo et al
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. This approach has been employed here to deconvolute the fibrillar structure shown in Fig
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. Manuscript approved for publication 2 March 2004 by the Scientific Editor
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. 4a), yet minimal staining observed in the pellets cultured in DMEM/ITS+/TGF-[beta]1 (Fig
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