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The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure
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The I [arrow right] A transition, which was referred to as the dominant unfolding process above, began at around 70°C and the midpoint of the transition (T^sub 1/2^) occurred at ~84°C (see Table 1)
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Excitation wavelength was 370 nm, and the emission intensity was collected from 430 to 700 nm
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0 but only when the pH is increased to pH 7
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Underlined items in the "Abundance" column indicatethat some or all of the specimens were preserved and at least oneindividual was correctly identified as Pseudopercis semifasciata
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Morphometric and genetic identification of eggs of spring-spawning sciaenids in lower Chesapeake Bay
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. However, we cannot rule out that an alternative explanation to the titration curve is that the deprotonation of a histidine residue can be felt by a residue(s) far enough in the three-dimensional structure (see Fersht (45) and references therein) affecting its (their) environmcnt(s)
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. The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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.ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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