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. The precipitate was removed by centrifugation at 8000 rpm for 30 min
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. In fact, the existence of two distinct length scales is mandated by field theories
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. Based on these findings, it is tempting to suggest that the changes monitored by far-UV CD were due to the proximity of residues in the tertiary structure of the whole intact EIC^sup sc^ structure, but not of the isolated domains (and then, in those modeled in this work)
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. After eliminating the denaturing force the proteins are not able to fold back into their native (and functional) structure and the still unfolded proteins form (or just stay on with) aggregates
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. The increase in Na+ activity is rather steep compared to the moderate changes in activity between K+, Rb+, and Cs+
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. The unfolding of this β-sheet allows the water molecules to penetrate into the interior of the catalytic domain, which results in the transformation of some Class I fluorophores to Class II and a further inactivation of the enzyme
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. Inspection of the EIC^sup sc^ (Fig
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. In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig
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. Life after corpse engulfment: phagocytosis of apoptotic cells leads to VEGF secretion and cell growth
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. The ability of this model to fit the data indicates that the proteinprotein interactions under these conditions are predominantly repulsive
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.To further demonstrate the use of fluorescence anisotropy decay, we probed TTR aggregation kinetics using time-resolved anisotropy
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