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The Democrat-controlled Senate refused to go along with the plan, attempting unsuccessfully to add a drug benefit to the government-run Medicare program
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. Earlier studies have shown that this protein shows exceptionally high stability in terms of free energy of unfolding when compared to other proteins from the same family
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. This widelycited result provides the theoretical basis for the assertion that denatured proteins are Flory-like random coils (3,4,11,12,17,37,30)
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. The values we use for σ^sub ij^ and ε^sub ij^ have been published previously (48)
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. 8 C suggest that the "observed" correlation between m values and ΔC^sub P^ to changes in solvent-accessible surface area might in fact be serendipitous
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. Then, the experiments monitoring protein stability under different conditions were carried out at protein concentrations ≥4 µM
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. Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli
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. Effects of cavity-forming mutations on the internal dynamics of Azurin
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. Indeed, engineered HuBChE with a reduced degree of glycosylation displays catalytic properties similar to those of the wild-type enzyme (62)
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. It was also observed that larger values of immersion depth resulted in a decrease of the wetting force and corresponding meniscus height, whereas the increase in substrate perimeter enhanced the wettability
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. 0, when the protein carries a net negative charge, can be described by hard sphere behavior those at pH 3
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. Funnels, pathways and the energy landscape of protein folding
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. 55% of the stations in densities that varied between two and 74 larvae/10 [m
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. , 1992) and, more recently, for Prolatilus jugularis (Ve1ez et al
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. Comprehensive structural insight is, however, limited by the intrinsic flexibility of the system
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. [FIGURE 10 OMITTED]An increase in absolute fecundity with age was observed in both prefertilization and postfertilization females, but they occurred at different rates
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. As illustrated in Figure 8, the absolute fecundity for a postfertilization 6-year-old black rockfish was only 58% of the estimated absolute fecundity for a prefertilizalion fish of the same age
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. Our results highlight the varying contributions of preformed secondary structure elements to tertiary folding of the hairpin ribozyme
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. Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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. Journal of Personality and Social Psychology, 32(2), 311-328
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.9 ml/min^sup -1^, with intrinsic fluorescence detection at 315 nm (excitation at 280 nm)
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. The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph
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. Local stiffness is typically quantified in terms of a persistence length, which is the length scale over which the chain behaves like a rigid rod (40,79)
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. Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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. Steady-state measurements Spectra were acquired in a 0
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. (58)PPAR[gamma], agonists enhance efferocytosis by human alveolar macrophages through the increased expression of CD36
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. Tjandra and co-workers (3) have shown, via ^sup 15^N-relaxation at multiple fields, that in free CaM the two domains undergo a slow "wobbling" motion on a timescale of ~3 ns, apparently not evident in target-bound CaM
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.Ralf Jansen,* Wojciech Dzwolak,[dagger] and Roland Winter** University of Dortmund, Department of Chemistry, Dortmund, Germany; and [dagger] High Pressure Research Center, Polish Academy of Sciences, Warsaw, PolandSubmitted July 1, 2004, and accepted for publication November 15, 2004
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. The difference in configurational entropy between two molecular conformations a and b can be estimated as ΔS = k^sub B^/2 ln(detσ^sub a^/detσ^sub b^), where σ^sub a^ and σ^sub b^, are the covariance matrices of atomic positional fluctuations of the two conformers and k^sub B^ is Boltzmann's constant
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. Journal of Raptor Research 25:140-141
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