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. There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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. To aid comparing the ANS binding propensity among the various proteins Table 2 lists the minimum number of binding sites (n) as well as the ratio n/Mw, normalization by the molecular weight taking into account that larger macromolecules, such as oligomers, have a greater probability to bind ANS simply for their larger mass
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. Approximately half of the HuBChE was inhibited with soman, as described by Masson and Goasdoue (35), before dialysis and lyophilization
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. Dynamic light scattering studies with rSBA and gSBA To determine the oligomeric stale of the intermediate, we measured the hydrodynamic radii of rSBA at different concentrations of the denaturant
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. Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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