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The recombinant form exhibits a 26°C lower T^sub m^ when compared to the glycosylated form
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The detailed dry meal was extracted with 20 m M phosphate buffer
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So our spectra are consistent with the assumption that all of the cytoplasmic diminazene would have been bound to macromolecules and thus give no MR signal
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Among these, the wetting balance test is considered the most versatile method8 because it is suited for obtaining quantitative information on the wettability of specimens of any shape and dimensions
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This is based on the relative small differences in chemical shifts between free and SEF2-1mp bound CaM and the low root mean square deviation of 1
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0 M NaCl at pH 5
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ANS shows a significant burst amplitude (A-state formation) followed by a slow phase in response to oligomeric aggregates
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In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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3 B) as described in the Materials and Methods section
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