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Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown) california home loan mortgage second southern .
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The mean residue ellipticity of the recombinant protein was similar to that of the wild-type, suggesting that there are no major structural changes between the native glycosylated and recombinant nonglycosylated proteins (Fig california home loan mortgage second southern .
To assay conformational preferences of highly denatured proteins, we quantify a variety of properties for EV-limit ensembles of 23 two-state proteins california home loan mortgage second southern .
The goal in these theories is to explain why chain properties such as R^sub g^, R^sub e^, R^sub h^, II, B^sub 2^, scattering functions, and internal correlations obey nontrivial power laws in the EV limit (39) california home loan mortgage second southern .
This choice is based on previous work (48) california home loan mortgage second southern .
Computational simulation of the statistical properties of unfolded proteins california home loan mortgage second southern .
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To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles california home loan mortgage second southern .
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