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5 and 3 M, and that a predissociated state with an expanded hydrodynamic radius compared to the native dimer is stabilized at 2
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INTRODUCTION Aminoacylase, a family of metalloenzymes found in many mammalian tissues and in microorganisms, catalyzes the stereospecific hydrolysis of acylamino acids to yield the corresponding organic acid and amino acid (1-3)
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Since ACYI can maintain its dimeric structure at a high GdnHCl concentration of 2 M (18), it seems that the dimerization domain was quite stable against denaturation
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The availability of an accurate theoretical framework for explaining scale-invariant properties of polymers in the EV limit has important ramifications for developing accurate theoretical descriptions for denatured proteins
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Thermal denaturation measurements Thermal unfolding curves were determined in the presence of 1 mM β-mercaptoethanol by following the emission fluorescence at 315, 335
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Endothelial cell death and decreased expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 in emphysema
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13 For such activities, WS flux showed the best wetting for Ni substrate, even having higher solder-flux interfacial tension (415 mN/mm) than NC flux
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Ca^sup 2+^ signaling can inhibit the transcriptional activities in vivo of the bHLH proteins E12 and SEF2-1 through direct binding of Ca^sup 2+^/CaM to the basic sequence of the proteins, resulting in inhibition of their DNA binding (15)
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The specific signal obtained from ThT binding to amyloid fibrils should therefore be a combined effect of decreased rotational relaxation and decreased polarity of the surrounding environment
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The formation of the globule itself from the denatured state corresponds in this picture to the first hypergutter in a series
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A lookup table was developed from this experiment and included with the part program
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At each sampling time, the state of the system is queried, and the recorded photon is assigned to either donor or acceptor channel, with a probability based on the FRET efficiency of the detected system state
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A 100 µl aliquot of both the samples and standards was pipetted into a 96 well plate
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The blood vessels in and around tumors tend to have larger-than-normal pores, so the liposomes pass into and accumulate in cancerous tissues more readily than they do in healthy ones
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Reed, who also serves as Southeast regional chairman for the Bush-Cheney campaign, received the fees from two lobbyists whose ties to the Indian tribes are now the subject of a federal investigation
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ConclusionsIn conclusion, it is remarkable that (possible) gender effects on motivation to gamble have not been previously reported as any gender differences found might offer additional insight to the primary motivation for both initial and sustained participation
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Journal of Social Psychology, 133, 507-511
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Great expectations: The legitimization of gambling in America, 1964-1995
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), The study of behavior (pp
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Should you draw another card or stand on 12?Probability should determine your decision, but psychology plays a major role
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