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. But the complexity and sophistication of the efforts that have been needed to reach this resolution show how, at times, a simple question can open up penetrating new leads into the labyrinth of evolutionary theory
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. Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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. The phase diagram analysis, which is a sensitive tool to detect folding intermediates (35), was performed to characterize whether an intermediate was involved in ACYI thermal unfolding
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. In these studies, we observe that mostly the mannose residues are involved in the intersubunit interactions
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. CONCLUSIONS In summary, our work shows that the nonglycosylated form of SBA unfolds via a non-two-state pathway, in contrast to the glycosykued naturally occurring protein
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. The second term provides an estimate of the conformational entropy, which opposes chain swelling
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. Our in vivo ^sup 1^H spectra did not reveal metabolites other than triglycerides
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. Competitive electrostatic binding of charged ligands to polyelectrolytes: planar and cylindrical geometries
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. For the high-resolution imaging, the AFM head (with optical block and base) was placed atop a commercially available active (piezo-actuated) vibrations-damping desk (from Halcyonics, Göttingen, Germany)
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. 1 g: 5 (often also 10-15 or sometimes even >20) fibers with diverse characteristics (like tubular, twisted, or undulated forms) and diameters generate both tight- and loose-plait ropelike forms (diameter: 5-25 nm) with endings fraying in single fibers
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. Recently Andricioaei and Karplus (2001) revised the quasiharmonic approach to allow for the use of Cartesian coordinates
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. We followed here a similar procedure to estimate errors on the conformational entropy changes upon complex formation for the sets of atoms that are involved in intermolecular interactions and/or show conformational changes (Table 1)
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. We determined stage-specific fecundity in black rockfish for females with unfertilized yolked oocytes (n=184) and fertilized eggs (n=85)
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. They showed that the scaling of R^sub g^ with N follows a power law of the form R^sub g^ = R^sub o^N^sup 0
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. Second, unlike hard-sphere potentials, which stipulate that all sterically allowed conformations are isoenergetic, soft-core potentials encode the requisite conformational specificity (48,53,58)
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. They are active as dimers, where the two basic regions bind DNA symmetrically as α-helices on opposite sides in the major groove
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. The sample was thereafter stained using 2% uranyl acetate for 20 s, and the grids were dried overnight
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. We note that we have found that the affinity between these proteins does not depend on the lipid phase (L
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. The mixture were then injected subcutaneously into BALB/c female mice (4 weeks of age)
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.Although trFRET and diffusion spFRET provide a consistent view of the overall tertiary folding of the hairpin ribozyme, spFRET is more direct and can provide additional information
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