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. To better understand the step-wise changes of the different Trp fluorophores, the spectrum recorded at each temperature was fitted using the discrete states model of Trp residues in proteins (30,32,34)
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. We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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. This value of E^sub a^ is comparable to the activation energy for the slow phase of apoA-2:DMPC disk denaturation (Fig
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. For instance, we have shown recently that, under certain conditions, the in vitro amyloidogenesis of insulin closely mimics certain aspects of the strain behavior of prion propagation (Dzwolak et al
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. The insulin monomer consists of two chains: a 21-residue-long A-chain and a 30-residue-long B-chain
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. An example shown in Fig
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. AcknowledgmentsThis study resulted from cooperative efforts of the USGS Biological Survey Unit and the Department of Zoology, National Museum of Natural History
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. PEURACH11 USGS Patuxent Wildlife Research Center, MRC-111, National Museum of Natural History, Washington, DC 20013
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. Sulphated glycosaminoglycan (GAG) content in the samples was assessed using the 1,9-dimethylmethylene blue (DMB) technique as described by Farndale, Sayers and Barrett
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