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Yeast GR presents a quite blue-shifted fluorescence emission spectrum (maximal emission wavelength at 317 nm for the native protein; see Fig california equity home loan third .
The excitation wavelength was 285 nm and emission was measured at 315 nm california equity home loan third .
5 and Bessel smoothing of 70%, and second derivative was carried out using the algorithm in the software with a nine-point Savitzky-Golay smoothing california equity home loan third .
Thus the intrinsic Trp fluorescence was used to investigate the changes of the tertiary structure of ACYI during thermal denaturation california equity home loan third .
The conformational stability and structure of the EI from Streptomyces coelicolor, EI^sup SC^, were explored in the absence and in the presence of its effectors by using several biophysical probes (namely, fluorescence, far-ultraviolet circular dichroism, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry) and computational approaches california equity home loan third .
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Indeed, if one compares liquid and frozen state profiles at the same effective ANS concentration in the liquid phase (bulk solution versus liquid water pools formed at intergranular spaces), we see that strong ANS binding is largely abolished in ice california equity home loan third .
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Moreover, the Mg^sup 2+^-dependent docking efficiencies of 2WJ, 3WJ, and 4WJ ribozyme constructs determined by trFRET are generally in accord with the results shown in Fig california equity home loan third .
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