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The excitation wavelength was 285 nm and emission was measured at 315 nm california directory.info home loan site .
These result confirmed the previous assumption that the irreversible inactivation of ACYI at high temperatures might be due to protein aggregation (25) california directory.info home loan site .
The amount of aggregated material, or the composition of the coexisting phases, was measured by centrifuging the samples for 5 min at 4000 g, removal of the upper phase, and determination of protein content of the aggregates or the concentration in each coexisting phase using spectrophotometry california directory.info home loan site .
By using model disks comprised of apoC-1 and DMPC, we showed that the heat-induced protein unfolding is a slow thermodynamically irreversible (or non-equilibrium) transition california directory.info home loan site .
The CD melting curves at 222 nm, Θ^sub 222^(T), recorded during sample heating and cooling from 1 to 80°C, show that the helical structure reaches maximum at 25°C and completely unfolds upon heating as well as cooling from 25°C (Fig california directory.info home loan site .
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