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. Despite the large amounts of ACYI that exist in mammalian kidney and liver, the reactivation of ACYI is particularly difficult in vitro (19-21), which suggests that the folding of ACYI in vivo might be assisted by the possible intracellular molecular chaperones and/or osmolytes
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. Differential scanning calorimetry Differential scanning calorimetry (DSC) experiments were performed with a VP-DSC differential scanning calorimeter (MicroCal
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. Steady-state fluorescenceContinuous wave steady-state fluorescence spectra were recorded using a Hitachi F-4500 spectroflurometer equipped with a Xe-lamp and a thermostatic cell holder
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. The first is that special tuning of nonnative interactions may contribute significantly to rapid folding; they stabilize the hypergutter-potentials that keep diffusive search dimensions under control
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. On the other hand, at acidic pH, EI^sup sc^ lost its tertiary structure and solvent-exposed hydrophobic patches, as suggested by 1), the changes in the fluorescence intensity (Fig
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.ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in IceGabellieri, EdiABSTRACT Freeze-induced perturbations of the protein native fold are poorly understood owing to the difficulty of monitoring their structure in ice
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. The specific properties of Li+ ion have been discussed previously (Wolf et al
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