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Despite the large amounts of ACYI that exist in mammalian kidney and liver, the reactivation of ACYI is particularly difficult in vitro (19-21), which suggests that the folding of ACYI in vivo might be assisted by the possible intracellular molecular chaperones and/or osmolytes california construction home loan .
Beyond this length scale, all internal distances scale with sequence separation in accordance with the standard power law for proteins in a good solvent california construction home loan .
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Differential scanning calorimetry Differential scanning calorimetry (DSC) experiments were performed with a VP-DSC differential scanning calorimeter (MicroCal california construction home loan .
Besides the disappearance of secondary structure elements, in most cases the three-dimensional structures are substantially altered california construction home loan .
Partition coefficient calculation The partition coefficients were calculated with the ClogP program (available at http://www california construction home loan .
5a-c for Ni substrate california construction home loan .
9 (the root mean square (rms) error in ΔH^sup #^ is ~4 kcal/mol, based on the rms error in f of ~0 california construction home loan .
Steady-state fluorescenceContinuous wave steady-state fluorescence spectra were recorded using a Hitachi F-4500 spectroflurometer equipped with a Xe-lamp and a thermostatic cell holder california construction home loan .
The first is that special tuning of nonnative interactions may contribute significantly to rapid folding; they stabilize the hypergutter-potentials that keep diffusive search dimensions under control california construction home loan .
The GROMACS program package (Lindahl et al california construction home loan .
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