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. These result confirmed the previous assumption that the irreversible inactivation of ACYI at high temperatures might be due to protein aggregation (25)
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. 7 kcal mol^sup -1^ in terms of free energy of unfolding (27)
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. 5 [Angstrom] at the average location of the center of mass
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. Inasmuch as sequence-specific effects are present over five- to nine-residue stretches, it is conceivable that there are native-like local biases as well as default biases for conformations such as polyproline II (48)
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. Samples of EI^sup sc^ at a final concentration of 44
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. We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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. Furthermore, these data correlate nicely to the crosslinking data of Fig
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. This suggests that, for these sets, sufficient sampling of conformational space has been achieved and that relatively precise conformational entropy differences between the two states can be obtained, although the values for the V3 loop should be taken with caution
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. However, it was also found that if the cells in monolayer were cultured in medium supplemented with FCS for one further passage then the cells would subsequently form pellets in the DMEM/10%FCS medium
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. Estimating the impact of RP on expenditure (price x quantity) is more difficult because there are more potential confounders in this case than if we were estimating the impact of RP on price alone
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. The Student-Life Stress Inventory was used in this study since it's potential respondent burden was acceptable, it was easy to self-administer and it had an acceptable previously reported internal consistency estimate (Cronbach's alpha = 0
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. The relationship between Student-Life Stress Inventory and mental component (MCS-12) HRQOL score was negative and significant at the p< 0
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. the enzyme was more resistant to proteolysis (open and solid circles) (Fig
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. Looking at the first portion of the righthand side of Eq
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. The second DSC peak is centered at 84°C, which is similar to the apparent T^sub m^ determined from the peak position in the first derivative of the CD melting data, dΘ^sub 222^(T)/dT (Fig
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. A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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