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.ACKNOWLEDGMENT: The authors thank Dr
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. We interpret these results such that although diminazene penetrates the cell membrane earlier than aceturate due to higher permeability, a large portion of it is strongly bound, and only a small portion of diminazene molecules weakly bound to nuclear macromolecules gives the initial signal
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.Summary of the use of ANS, Bis-ANS, DCVJ, and ThT as fluorescence conformational probesThe results of the analysis of the fluorescence response in binding to unfolded monomers, native TTR, and various stages of the aggregation reaction is summarized in Table 2
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